1. Optical microscopy: basic physics and microscope configurations.
2. Laser scanning and spinning disk confocal microscopy.
3. Sub-nanometer spatial resolution by using fluorescence resonance energy transfer (FRET) technique.
4. Multi-photon excitation. 4Pi microscopy. Image interference microscopy (I2M, I3M and I5M).
5. Structured-illumination microscopy. Saturated structured-illumination microscopy (SSIM).
6. Fluorescence lifetime imaging (FLIM). Dark-state relaxation microscopy (D-Rex or T-Rex). Dynamic saturation optical microscopy (DSOM).
7. Fluorescence imaging with one-nanometer accuracy (FIONA). Single-molecule high-resolution colocalization (SHREC).
8. Stochastic optical reconstruction microscopy (STORM).
9. Photoactivation or photoswitching localization microscopy (PALM or FPALM).
10. Stimulated emission depletion (STED). Reversible saturable optical (fluorescence) transition microscopy (RESOLFT).
