Hauptseminar: Lichtoptische Nanoskopie

  • Typ: HS
  • Lehrstuhl: Fakultät für Physik
  • Semester: Wintersemester 2009/2010
  • Dozent:
    Andrei Kobitski
  • SWS: 2
  • LVNr.: 2103014
  • Neue Methoden der lichtoptischen Mikroskopie ermöglichen die Beobachtung von biologischen Prozessen in lebenden Zellen mit Auflösungen im Bereich von 10 – 50 nm.

     

    Themen

    1.  Optical microscopy: point spread function and Abbe law. Microscope configurations: confocal, epifluorescence and total internal reflection.

    2.  Spinning disk confocal microscopy. Light sheet illumination.

    3.  Multi-photon excitation. 4Pi microscopy. Image interference microscopy (I2M, I3M and I5M).

    4.  Structured-illumination microscopy (SIM). Saturated structured-illumination microscopy (SSIM). Dynamic saturation optical microscopy (DSOM).

    5.  Stimulated emission depletion (STED). Reversible saturable optical (fluorescence) transition microscopy (RESOLFT).

    6.  Fluorescence imaging with one-nanometer accuracy (FIONA). Single-molecule high-resolution colocalization (SHREC).

    7.  Stochastic optical reconstruction microscopy (STORM).

    8.  Photoactivation or photoswitching localization microscopy (PALM or FPALM).

    9.  Fluorescence resonance energy transfer (FRET) technique.

    10.  Fluorescence lifetime imaging (FLIM). Dark-state relaxation microscopy (D-Rex or T-Rex).

     

 

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Neue Methoden der lichtoptischen Mikroskopie ermöglichen die Beobachtung von biologischen Prozessen in lebenden Zellen mit Auflösungen im Bereich von 10 – 50 nm.

 

Themen

1.  Optical microscopy: point spread function and Abbe law. Microscope configurations: confocal, epifluorescence and total internal reflection.

2.  Spinning disk confocal microscopy. Light sheet illumination.

3.  Multi-photon excitation. 4Pi microscopy. Image interference microscopy (I2M, I3M and I5M).

4.  Structured-illumination microscopy (SIM). Saturated structured-illumination microscopy (SSIM). Dynamic saturation optical microscopy (DSOM).

5.  Stimulated emission depletion (STED). Reversible saturable optical (fluorescence) transition microscopy (RESOLFT).

6.  Fluorescence imaging with one-nanometer accuracy (FIONA). Single-molecule high-resolution colocalization (SHREC).

7.  Stochastic optical reconstruction microscopy (STORM).

8.  Photoactivation or photoswitching localization microscopy (PALM or FPALM).

9.  Fluorescence resonance energy transfer (FRET) technique.

10.  Fluorescence lifetime imaging (FLIM). Dark-state relaxation microscopy (D-Rex or T-Rex).