Institut für Angewandte Physik - Arbeitsgruppe Prof. Dr. Nienhaus

HS "Lichtoptische Nanoskopie"

  1. Basic physics of optical microscopy. Confocal and wide-field microscope configuration. Single-molecule detection.
  2. 3D imaging techniques. Laser spinning disk confocal microscopy. Light-sheet microscopy.
  3. Localization microscopy. Fluorescence imaging with one-nanometer accuracy (FIONA). Stochastic optical reconstruction microscopy (STORM) and Photoactivation or photoswitching localization microscopy (PALM or FPALM).
  4. Stimulated emission depletion (STED).
  5. Sub-nanometer spatial resolution by using fluorescence resonance energy transfer (FRET) technique.