HS "Lichtoptische Nanoskopie"

Hauptseminar im Wintersemester

Neue Methoden der lichtoptischen Mikroskopie ermöglichen die Beobachtung von biologischen Prozessen in lebenden Zellen mit Auflösungen im Bereich von 10 – 50 nm.

Themen

1. Basic physics of optical microscopy. Confocal and wide-field microscope configuration. Single-molecule detection.
2. 3D imaging techniques. Laser spinning disk confocal microscopy. Light-sheet microscopy.
3. Localization microscopy. Fluorescence imaging with one-nanometer accuracy (FIONA). Stochastic optical reconstruction microscopy (STORM) and Photoactivation or photoswitching localization microscopy (PALM or FPALM).
4. Stimulated emission depletion (STED).
5. Sub-nanometer spatial resolution by using fluorescence resonance energy transfer (FRET) technique.