Photo-Induced Click Chemistry for DNA Surface Structuring by Direct Laser Writing
A. Kerbs, P. Mueller, M. Kaupp, I. Ahmed, A.S. Quick, D. Abt, M. Wegener, C.M. Niemeyer, C. Barner-Kowollik, and L. Fruk
Chem. Eur. J. 23, 4990 - 4994 (2017)
- Date: 27.03.2017
Oligonucleotides containing photo-caged dienes were prepared and shown to react quantitatively in a light-induced Diels–Alder cycloaddition with functional maleimides in aqueous solution within minutes. Due to its high yield and fast rate, the reaction was exploited for DNA surface patterning with sub-micrometer resolution employing direct laser writing (DLW). Functional DNA arrays were written by direct laser writing (DLW) in variable patterns, which were further encoded with fluorophores and proteins through DNA directed immobilization. This mild and efficient light-driven platform technology holds promise for the fabrication of complex bioarrays with sub-micron resolution.