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Near-field optical fluorescence correlation spectroscopy

Near-field optical fluorescence correlation spectroscopy
Autor:

 J. Pérez, M. Herrmann, D. Gradl, and A. Naber

Links:
Quelle:

Proc. SPIE, Vol. 7571, 757118 (2010)

Datum: 25.02.2010

Abstract:

The application of Fluorescence Correlation Spectroscopy (FCS) to Near-field Scanning Optical Microscopy on diffusing molecules in solution is presented. The ultra small excitation volume in such a technique allows measurements with fluorophore concentrations of up to 10μM, making FCS in biological environment at nearly native conditions possible.