Near-field optical fluorescence correlation spectroscopy

  • Author:

     J. Pérez, M. Herrmann, D. Gradl, and A. Naber

  • Source:

    Proc. SPIE, Vol. 7571, 757118 (2010)

  • Date: 25.02.2010
  • Abstract:

    The application of Fluorescence Correlation Spectroscopy (FCS) to Near-field Scanning Optical Microscopy on diffusing molecules in solution is presented. The ultra small excitation volume in such a technique allows measurements with fluorophore concentrations of up to 10μM, making FCS in biological environment at nearly native conditions possible.